Composite

Part:BBa_K3578011:Design

Designed by: Ruyi Shi   Group: iGEM20_Worldshaper-Nanjing   (2020-09-20)


AnAmyA Starch degradation


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3257
    Illegal EcoRI site found at 3307
    Illegal EcoRI site found at 4269
    Illegal SpeI site found at 756
    Illegal PstI site found at 1260
    Illegal PstI site found at 1573
    Illegal PstI site found at 2572
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3257
    Illegal EcoRI site found at 3307
    Illegal EcoRI site found at 4269
    Illegal NheI site found at 817
    Illegal SpeI site found at 756
    Illegal PstI site found at 1260
    Illegal PstI site found at 1573
    Illegal PstI site found at 2572
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3257
    Illegal EcoRI site found at 3307
    Illegal EcoRI site found at 4269
    Illegal BglII site found at 1808
    Illegal BglII site found at 2704
    Illegal BglII site found at 5538
    Illegal BamHI site found at 2731
    Illegal XhoI site found at 2665
    Illegal XhoI site found at 4252
    Illegal XhoI site found at 4281
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3257
    Illegal EcoRI site found at 3307
    Illegal EcoRI site found at 4269
    Illegal SpeI site found at 756
    Illegal PstI site found at 1260
    Illegal PstI site found at 1573
    Illegal PstI site found at 2572
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3257
    Illegal EcoRI site found at 3307
    Illegal EcoRI site found at 4269
    Illegal SpeI site found at 756
    Illegal PstI site found at 1260
    Illegal PstI site found at 1573
    Illegal PstI site found at 2572
    Illegal NgoMIV site found at 3395
    Illegal AgeI site found at 1336
    Illegal AgeI site found at 2938
    Illegal AgeI site found at 4866
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1


Source

EXP, XPR2t were amplified from Yarrowia lipolytica. Leu marker was synthesized chemically by TSINGKE Biotech Co., Ltd., China. AnAmyA was codon-optimized and synthesized chemically by TSINGKE Biotech Co., Ltd., China.

References